Isolation And Characterization of Isaria Farinosa from Iraqi Gara Mountain Soil Using Morphological And Molecular Approaches

Isaria farinosa, formerly known as Paecilomyces farinosus, have a worldwide distribution and relatively wide host range. The aim of the work is to isolate identify fungus from soil Gara mountain using molecular analysis morphological characteristics species were isolated samples collected at an insect overwintering sites in mountain, Kurdistan Region, Iraq selective medium Oat culture modified with CTAB cyclohexamide. Results demonstrated that conidia are globose subglobose ellipsoidal shape, 2-2.3 x 1-1.3 um size, hyaline smooth walls. Besides, PCR based method revealed sequences high level (100%) sequence homology I. farinosa. present study provides better insight into related entomopathogenic fungi terms isolation, morphology phylogeny